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Home/Compounds/Research Reagent/5-bromo-4-chloroindol-3-yl β-D-galactopyranoside
C14H15BrClNO6
408.63 g/mol
Research ReagentWarning

X-Gal

CAS7240-90-6EC230-640-8
Category
Research Reagent
Compound Class
Heterocycle
Primary Use
Research Compound

X-Gal (5-bromo-4-chloro-3-indolyl beta-D-galactoside) is a chromogenic compound used as a substrate for the enzyme beta-galactosidase. Upon enzymatic cleavage, it produces an intensely blue product, enabling visual detection of enzyme activity in laboratory and research settings.

Chromogenic substrate for beta-galactosidase in histological stainingIndicator in bacterial cloning experiments

Content scope: Information here supports chemical identification and industrial supply, research, and manufacturing contexts. It is not a therapeutic claim, medical advice, or drug-use instructions.

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Safety Reference

Harmful
Harmful
Warning
H302Harmful if swallowed
H312Harmful in contact with skin
H332Harmful if inhaled
Identifiers
IUPAC Name

(2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol

InChIKey

OPIFSICVWOWJMJ-AEOCFKNESA-N

SMILES

C1=CC(=C(C2=C1NC=C2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br

Synonyms (31)
  • BCIG
  • X-Gal