C40H30N10O62
746.7 g/mol
Research ReagentWarning
NITROBLUE TETRAZOLIUM
CAS298-83-9EC206-067-4
Category
Research Reagent
Compound Class
Heterocycle
Primary Use
Research Compound
Nitro blue tetrazolium is a chemical compound consisting of two tetrazole moieties. It is widely used as a histochemical stain for dehydrogenases and in immunology for the sensitive detection of alkaline phosphatase, where it serves as the oxidant in combination with BCIP.
Histochemical stain for dehydrogenasesOxidant in alkaline phosphatase detection systems
Content scope: Information here supports chemical identification and industrial supply, research, and manufacturing contexts. It is not a therapeutic claim, medical advice, or drug-use instructions.
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Related Compounds
ReferenceSame Category
Triphenyltetrazolium chloride
CAS298-96-4
research compound
ReferenceSame Category
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ReferenceSame Category
2-bromoisonicotinamide
CAS29840-73-1
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ReferenceSame Category
(1S,2S)-(-)-1,2-Diphenyl-1,2-ethanediamine
CAS29841-69-8
research compound
Safety Reference
Warning
H302Harmful if swallowed
H312Harmful in contact with skin
H315Causes skin irritation
H319Causes serious eye irritation
H332Harmful if inhaled
H335May cause respiratory irritation
Customs Tariff Classification
The international HS code for NITROBLUE TETRAZOLIUM (CAS 298-83-9) is 2933.99.
HS code (international, 6-digit)
2933.99
EU CN code (8-digit)
2933 99 80
Classification per the EU customs inventory ECICS (2026-07 snapshot, HS 2022). National tariff lines and product form can affect the final classification.
Identifiers
IUPAC Name
2-[2-methoxy-4-[3-methoxy-4-[3-(4-nitrophenyl)-5-phenyltetrazol-2-ium-2-yl]phenyl]phenyl]-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium
InChIKey
JPXMTWWFLBLUCD-UHFFFAOYSA-N
SMILES
COC1=C(C=CC(=C1)C2=CC(=C(C=C2)[N+]3=NC(=NN3C4=CC=C(C=C4)[N+](=O)[O-])C5=CC=CC=C5)OC)[N+]6=NC(=NN6C7=CC=C(C=C7)[N+](=O)[O-])C8=CC=CC=C8
Synonyms (19)
- Blue, Nitrotetrazolium
- NBT (dye)
- NITROBLUE TETRAZOLIUM
- Nitro tetrazolium BT
- Nitroblue tetrazolium cation
- Nitroblue tetrazolium ion
- Nitroblue tetrazolium salt
- Nitrotetrazolium Blue
- Tetrazolium Nitro Blue
- Tetrazolium nitro BT
- nitro blue tetrazolium(2+)
- nitro bt
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