Ac-WEHD-AFC is a fluorogenic substrate specifically designed for caspase assays, commonly used in apoptosis research. It contains a tetrapeptide sequence (Ac-WEHD) linked to a fluorescent AFC (7-amino-4-trifluoromethylcoumarin) reporter group, which upon enzymatic cleavage releases a fluorescent signal.
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(4S)-4-[[(2S)-2-acetamido-3-(1H-indol-3-yl)propanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
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